Characterization of DNA-binding sequences for CcaR in the cephamycin-clavulanic acid supercluster of Streptomyces clavuligerus
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منابع مشابه
Transcriptomic analysis of Streptomyces clavuligerus ΔccaR::tsr: effects of the cephamycin C-clavulanic acid cluster regulator CcaR on global regulation*
Streptomyces clavuligerus ATCC 27064 and S. clavuligerus ΔccaR::tsr cultures were grown in asparagine-starch medium, and samples were taken in the exponential and stationary growth phases. Transcriptomic analysis showed that the expression of 186 genes was altered in the ccaR-deleted mutant. These genes belong to the cephamycin C gene cluster, clavulanic acid gene cluster, clavams, holomycin, d...
متن کاملCcaR is an autoregulatory protein that binds to the ccaR and cefD-cmcI promoters of the cephamycin C-clavulanic acid cluster in Streptomyces clavuligerus.
The putative regulatory CcaR protein, which is encoded in the beta-lactam supercluster of Streptomyces clavuligerus, has been partially purified by ammonium sulfate precipitation and heparin affinity chromatography. In addition, it was expressed in Escherichia coli, purified as a His-tagged recombinant protein (rCcaR), and used to raise anti-rCcaR antibodies. The partially purified CcaR protein...
متن کاملOverproduction of Clavulanic Acid by UV Mutagenesis of Streptomyces clavuligerus
Clavulanic acid is produced industrially by fermentation of Streptomyces clavuligerus and researches have increased its production by strain improvement, recombinant DNA technology, and media composition and growth condition optimization. The main objective of this study was to increase the level of clavulanic acid production from Streptomyces clavuligerus (DSM 738), using UV irradiation. After...
متن کاملStudies on the Biosynthetic Pathways of Clavulanic Acid and Cephamycin C in Streptomyces clavuligerus
Mackenzie, A., Studies on the Biosynthetic Pathways of Clavulanic Acid and Cephamycin C in Streptomyces clavuligerus. Doctoral dissertation. ISSN 1652-6880 ISBN 978-91-576-7318-3 The discovery of penicillin, a β-lactam antibiotic, changed the way humans thought about infectious disease. Unfortunately, the widespread use of antibiotics has lead to a concomitant increase in bacterial resistance t...
متن کاملDissociation of cephamycin C and clavulanic acid biosynthesis by 1,3-diaminopropane in Streptomyces clavuligerus.
Streptomyces clavuligerus produces simultaneously cephamycin C (CephC) and clavulanic acid (CA). Adding 1,3-diaminopropane to culture medium stimulates production of beta-lactam antibiotics. However, there are no studies on the influence of this diamine on coordinated production of CephC and CA. This study indicates that 1,3-diaminopropane can dissociate CephC and CA productions. Results indica...
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ژورنال
عنوان ژورنال: Molecular Microbiology
سال: 2011
ISSN: 0950-382X
DOI: 10.1111/j.1365-2958.2011.07743.x